The nutrient deficiencies in plant leading to reduction in the yield and quality of crop. In high value crops, particularly horticulture crops, there are huge losses to the farmers. All these problems cannot be identified without testing and diagnosis of plant tissue. Through tissue testing it can easily understand the nutrient status in the plant. The nutrient content of a plant can be assessed by testing a sample of tissue from that plant. This, in turn, shows whether soil nutrient supplies are adequate. In addition, plant tissue analysis will detect unseen deficiencies and may confirm visual symptoms of deficiencies. The plant testing useful for calculating nutrient uptake by plant. The Krishi Vigyan Kendra, Kheda provide facility of analysis of leaf tissue. The total N, Fe, Mn, Zn and Cu are analyzed in Leaf Tissue Analysis Laboratory.